aroD [2019-07-05 11:33:11]

shikimate dehydrogenase

locus: BSU25660

pI: 6.08

mw: 30.49 kDa

protein length: 280 aa

gene length: 843 bp

function: biosynthesis of aromatic amino acids

product: shikimate dehydrogenase

essential: no

ec: 1.1.1.25

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.13|Biosynthesis/ acquisition of aromatic amino acids]

Gene

Coordinates: 2,644,630 2,645,472

The protein

Catalyzed reaction/ biological activity

• Shikimate + NADP⁺ = 3-dehydroshikimate + NADPH (according to Swiss-Prot)

Protein family

• shikimate dehydrogenase family (according to Swiss-Prot)

Structure

• [PDB|2HK8] (from ''Aquifex aeolicus'', 42% identity, 61% similarity) [Pubmed|17649975]

Expression and Regulation

Operons

• genes: [gene|722FD63050EED803C859FFD61338C02D5BB4CC1C|yqeG]-[gene|959C0ED7B9DA50F0EADCF1565405B878D36DA206|yqeH]-[gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]-[gene|7774FC36BAEAD33EDD48CB77DA0B5E7E0EA38CA2|yqeI]-[gene|AA4BB1F5B58371D536A6BBA86F6ECB5E5BFB12A8|nadD]-[gene|247C47AC70A0EA53E625871D9BC463A06E6B05FB|yqeK]-[gene|B9D5E9B566D28423232C1A28C966CDEA0E5A6B78|rsfS]-[gene|7F63251ABC07F6C11C57B62CBB948240B08D0181|yqeM]
  description: [Pubmed|22383849]

Biological materials

Mutant

• BKE25660 ([gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25660 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_TTTCATACACCTCTCCCCTT, downstream forward: _UP4_ATAGGAAAATTAGGAGGAAC
• BKK25660 ([gene|776D79C45DADA631D4A75B0773CFF471A87D9EEC|aroD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25660 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTCATACACCTCTCCCCTT, downstream forward: _UP4_ATAGGAAAATTAGGAGGAAC

References

• 6811271,24628944,22383849